PRODUCT SHEET

Holmium Oxide Solution (240 – 650 nm)

This Holmium Oxide Liquid Reference Material is intended for the qualification of wavelength calibration in the ultraviolet and visible spectral regions ranging from 240 nm to 650 nm. It is suitable for use with spectrophotometers having spectral bandwidths of 3 nm or less and is accepted by most pharmacopoeias and standardisation bodies.

The reference consists of a 4% m/v holmium oxide solution in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette. The use of holmium oxide dissolved in perchloric acid produces a stable Ho3+ aqueous species, resistant to variations in temperature and concentration, providing a reliable and reproducible wavelength standard for instrument qualification.

Features and Purposes
  • Spectral Range: 240 nm to 650 nm
  • Holmium Oxide Concentration: 4% m/v in 10% v/v perchloric acid solvent
  • Cuvette Material: 10 mm pathlength far UV quartz cuvette, permanently sealed by heat fusion
  • Number of Defined Peaks: 14 characteristic absorption peaks used for wavelength calibration
  • Peak Wavelengths Approximate Values (nm): 241, 250, 278, 288, 334, 346, 361, 385, 417, 451, 468, 485, 537, 641
  • Certified peak values are provided for various spectral bandwidths (0.10 to 5.00 nm) as indicated in the accompanying calibration certificate
  • Widely accepted by pharmacopoeias and standardisation bodies for spectrophotometer wavelength scale validation
DOCUMENTS AND RESOURCES
Certified Reference Materials for Spectroscopy 2024
Starna Cell Catalogue Micro Rectangular with Lid and Stopper
Holmium Oxide Liquid Reference for UV and Visible Spectrophotometer Wavelength Qualification
PRODUCT SHEET

Holmium Oxide Solution (240 – 650 nm)

This Holmium Oxide Liquid Reference Material is intended for the qualification of wavelength calibration in the ultraviolet and visible spectral regions ranging from 240 nm to 650 nm. It is suitable for use with spectrophotometers having spectral bandwidths of 3 nm or less and is accepted by most pharmacopoeias and standardisation bodies.

The reference consists of a 4% m/v holmium oxide solution in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette. The use of holmium oxide dissolved in perchloric acid produces a stable Ho3+ aqueous species, resistant to variations in temperature and concentration, providing a reliable and reproducible wavelength standard for instrument qualification.

Features and Purposes
  • Spectral Range: 240 nm to 650 nm
  • Holmium Oxide Concentration: 4% m/v in 10% v/v perchloric acid solvent
  • Cuvette Material: 10 mm pathlength far UV quartz cuvette, permanently sealed by heat fusion
  • Number of Defined Peaks: 14 characteristic absorption peaks used for wavelength calibration
  • Peak Wavelengths Approximate Values (nm): 241, 250, 278, 288, 334, 346, 361, 385, 417, 451, 468, 485, 537, 641
  • Certified peak values are provided for various spectral bandwidths (0.10 to 5.00 nm) as indicated in the accompanying calibration certificate
  • Widely accepted by pharmacopoeias and standardisation bodies for spectrophotometer wavelength scale validation
DOCUMENTS AND RESOURCES
Certified Reference Materials for Spectroscopy 2024
Starna Cell Catalogue Micro Rectangular with Lid and Stopper
Holmium Oxide Liquid Reference for UV and Visible Spectrophotometer Wavelength Qualification

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