PRODUCT SHEET

Toluene in Methanol (265 – 270 nm)

Toluene in methanol solution is provided as a 0.020% v/v mixture, permanently sealed by heat fusion in a far UV quartz cell. The package includes a methanol blank for spectral comparison.

This reference is designed for use in derivative UV-Visible spectroscopy, where absorption spectra are mathematically transformed into first, second, or higher order derivatives. This approach enhances resolution by differentiating overlapping peaks that appear as shoulders in the original absorption spectrum. The most common application is the second derivative (d2A/dλ2), which facilitates the identification of individual spectral components.

Features and Purposes
  • Concentration: 0.020% v/v toluene in methanol
  • Cell type: Far UV quartz cell, permanently sealed by heat fusion
  • Includes methanol blank for baseline correction
  • Intended use: Resolution test for derivative spectroscopy (UV-Visible)
  • Typical spectral features: In second derivative mode, a small negative extremum at 285 nm flanked by larger negative extrema at 261 nm and 268 nm
  • Performance criteria: Ratio A/B > 0.2 as per pharmacopoeial requirements
  • Catalogue number: RM-TM (toluene in methanol cell with methanol blank)
DOCUMENTS AND RESOURCES
Certified Reference Materials for Spectroscopy
Starna Cell Catalogue Micro Rectangular with Lid and Stopper
Toluene in Methanol Reference for UV Spectrophotometry Derivative Spectroscopy
PRODUCT SHEET

Toluene in Methanol (265 – 270 nm)

Toluene in methanol solution is provided as a 0.020% v/v mixture, permanently sealed by heat fusion in a far UV quartz cell. The package includes a methanol blank for spectral comparison.

This reference is designed for use in derivative UV-Visible spectroscopy, where absorption spectra are mathematically transformed into first, second, or higher order derivatives. This approach enhances resolution by differentiating overlapping peaks that appear as shoulders in the original absorption spectrum. The most common application is the second derivative (d2A/dλ2), which facilitates the identification of individual spectral components.

Features and Purposes
  • Concentration: 0.020% v/v toluene in methanol
  • Cell type: Far UV quartz cell, permanently sealed by heat fusion
  • Includes methanol blank for baseline correction
  • Intended use: Resolution test for derivative spectroscopy (UV-Visible)
  • Typical spectral features: In second derivative mode, a small negative extremum at 285 nm flanked by larger negative extrema at 261 nm and 268 nm
  • Performance criteria: Ratio A/B > 0.2 as per pharmacopoeial requirements
  • Catalogue number: RM-TM (toluene in methanol cell with methanol blank)
DOCUMENTS AND RESOURCES
Certified Reference Materials for Spectroscopy
Starna Cell Catalogue Micro Rectangular with Lid and Stopper
Toluene in Methanol Reference for UV Spectrophotometry Derivative Spectroscopy

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