Toluene in Hexane (265 - 270 nm)

PRODUCT SHEET

Toluene in Hexane (265 – 270 nm)

This Reference Material consists of two far-UV quartz cells: one containing a 0.020% v/v solution of toluene in hexane and the other serving as a blank filled with hexane only. Both cells are permanently sealed by heat fusion, ensuring sample integrity for spectral qualification.

It is specifically designed for qualifying the Spectral Bandwidth (SBW) of spectrophotometers in the ultraviolet region between 265 nm and 270 nm. The spectral bandwidth, representing the wavelength range emitted by the instrument’s monochromator exit slit, is critical for resolution—the instrument’s ability to distinguish between two adjacent absorbance peaks.

Features and Purposes

Intended spectral bandwidth qualification range: 265–270 nm in the ultraviolet region
Suitable for spectrophotometers with SBW of 3 nm or less
Comprises two far-UV quartz cells: one with toluene/hexane solution, one hexane blank
Solution concentration: 0.020% v/v toluene in hexane
Cells permanently sealed by heat fusion to prevent contamination and evaporation
Accepted by major Pharmacopoeias and Standardisation Bodies for spectral bandwidth qualification
Provides a peak absorbance at 269 nm and a minimum at 267 nm; the peak-to-trough absorbance ratio measures spectral resolution
Typical ratio values (at 20 °C) vary with SBW:
- 0.5 nm SBW: 2.4–2.5
- 1.0 nm SBW: 2.0–2.1
- 1.5 nm SBW: 1.6–1.7
- 2.0 nm SBW: 1.3–1.4
- 3.0 nm SBW: 1.0–1.1
Compliance recommendation: spectrophotometer SBW should not exceed 1.8 nm to meet Pharmacopoeial standards

DOCUMENTS AND RESOURCES

Certified Reference Materials for Spectroscopy

Starna Cell Catalogue Micro Rectangular Cells with Lid and Stopper

Toluene in Hexane Spectrophotometer UV Bandwidth Qualification Reference Material

PRODUCT SHEET