The product consists of a Rhodamine 101 solution in ethanol, sealed by heat fusion in a high-quality far-UV fluorometer cell. It is supplied together with an ethanol blank cell for baseline correction. This reference standard is designed for determining the fluorescence quantum yield (ΦF) of substances.
The fluorescence quantum yield represents the ratio of photons absorbed to photons emitted by fluorescence. The reference has a quantum yield of 1.0 at 25°C, enabling quantitative comparison. Its excitation range is approximately 460–600 nm and the emission range spans 550–700 nm; typically, a 535 nm excitation wavelength is recommended for most instruments. The solution’s blank-corrected absorbance is kept below literature recommended thresholds to minimize internal filter effects, ensuring accurate measurements.
The product consists of a Rhodamine 101 solution in ethanol, sealed by heat fusion in a high-quality far-UV fluorometer cell. It is supplied together with an ethanol blank cell for baseline correction. This reference standard is designed for determining the fluorescence quantum yield (ΦF) of substances.
The fluorescence quantum yield represents the ratio of photons absorbed to photons emitted by fluorescence. The reference has a quantum yield of 1.0 at 25°C, enabling quantitative comparison. Its excitation range is approximately 460–600 nm and the emission range spans 550–700 nm; typically, a 535 nm excitation wavelength is recommended for most instruments. The solution’s blank-corrected absorbance is kept below literature recommended thresholds to minimize internal filter effects, ensuring accurate measurements.
